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Item #:
51556604
Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL
Item #:
51556604
WST 141
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1X Phosphate Buffered Saline (PBS) is a balanced, isotonic salt solution used for a variety of cell culture applications.
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What Stands Out
Sterile Solution
Ensures contamination-free experiments, enhancing the reliability and accuracy of your research outcomes with ready-to-use phosphate buffered saline.
Convenient Volume
500 mL quantity provides ample supply for multiple applications, reducing the need for frequent reorders and saving laboratory time and resources.
Optimal pH Stability
Maintains a stable pH environment, crucial for biological experiments, ensuring consistent performance across various applications in cell culture and immunology.
Product Details
- Manufactured in USA in ISO 9001 and 13485 certified facility
- For research use only, not for human or animal clinical/therapeutic use
- Each production lot undergoes stringent Good Manufacturing Practices and quality control tests
- 1X Phosphate Buffered Saline (PBS) is 0.22 micron filter-sterilized
- Contains 137mM NaCl, 10mM NaH2PO4, 2.7mM KCl, 1.8mM KH2PO4 with pH 7.4
- Used for cell culture applications such as washing cells, transporting cells, and preparing reagents
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Laboratory Research
Ideal for researchers conducting cell culture, protein purification, and immunoassays requiring a stable pH environment.
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Diagnostic Testing
Useful for labs performing diagnostic tests, providing a sterile and buffered solution for sample preparation.
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Biological Experiments
Suitable for biological experiments where maintaining osmotic balance and pH stability is critical for results.
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Long-term Storage
Not suitable for long-term storage of cells or samples due to potential degradation and nutrient depletion.
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Non-scientific Use
Not recommended for any applications outside of laboratory settings or scientific research and experimentation.
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High Concentration Needs
May not be suitable for high-concentration scenarios where concentrated solutions are required for specific applications.
Product Description
Phosphate Buffered Saline, PBS (1X), Sterile, 500 mL
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is Phosphate Buffered Saline (PBS) used for?
Answer: Phosphate Buffered Saline (PBS) is primarily used in biological research as a buffer solution for dissolving biological samples. Its formulation helps maintain a stable pH, making it ideal for applications like cell culture, immunoassays, and molecular biology experiments. The sterile nature of PBS prevents contamination, ensuring accurate results. For example, in cell culture, PBS is frequently used to rinse cells before trypsinization, as it helps maintain osmotic balance and cell viability. -
Question:
Is PBS 1X suitable for use in cell culture?
Answer: Yes, PBS 1X is suitable for cell culture applications. It provides a balanced salt solution that supports the physiological conditions necessary for cell survival and growth. With its sterile formulation, PBS helps in processes such as washing cells, preparing samples for flow cytometry, and diluting other reagents. Many researchers use PBS to remove serum before enzymatic detachment of adherent cells, ensuring minimal disruption to experimental results. -
Question:
How should Phosphate Buffered Saline be stored?
Answer: Phosphate Buffered Saline should be stored at room temperature in a cool, dry place, away from direct sunlight. It is recommended to keep PBS tightly sealed in its original container to prevent contamination. While PBS can be stored for extended periods, it is advisable to check for any visible contaminants before use. Proper storage ensures the buffer retains its efficacy, which is critical for sensitive applications such as immunofluorescence staining. -
Question:
Can PBS be used in molecular biology techniques?
Answer: Yes, PBS is widely used in various molecular biology techniques, including PCR (Polymerase Chain Reaction), Western blotting, and ELISA (Enzyme-linked Immunosorbent Assay). Its role as a buffering agent aids in maintaining a stable pH during reactions, which is crucial for enzyme activity and specificity. For instance, during the dilution of antibodies or nucleic acids, PBS helps maintain the integrity of the reagents, contributing to reproducible experimental outcomes. -
Question:
Is PBS 1X compatible with mammalian cells?
Answer: PBS 1X is compatible with mammalian cells, as it mimics physiological conditions, making it an ideal medium for cell handling and experiments. Its isotonic nature prevents osmotic shock, ensuring cell health during procedures like trypsinization or washing. Researchers often utilize PBS when preparing mammalian cells for flow cytometry assays, as it aids in maintaining cell viability while optimizing staining protocols. -
Question:
What are the components of Phosphate Buffered Saline?
Answer: Phosphate Buffered Saline typically contains sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate. These components work together to create an isotonic and buffered environment, maintaining a stable pH around 7.4. This balanced formulation is crucial for biological applications, as it prevents changes in cellular environments that could disrupt normal function. Understanding these components can help researchers select the right medium for their specific experiments. -
Question:
Can I use PBS for preparing samples for flow cytometry?
Answer: Absolutely, PBS is a preferred buffer for preparing samples for flow cytometry. Its isotonic properties help preserve cell morphology and viability, which are essential for accurate analysis. By using PBS to dilute samples, researchers can effectively minimize background noise and improve the clarity of cytometric data. Additionally, many protocols recommend washing cells with PBS to reduce nonspecific binding of antibodies, enhancing the quality of results. -
Question:
Is the PBS 1X solution sterile?
Answer: Yes, PBS 1X is available in a sterile formulation, ensuring it is free from microbial contamination. This sterility is critical in laboratory settings where contamination can compromise experimental integrity. For example, using sterile PBS is essential when handling cultured cells or preparing immunological assays to prevent bacterial or fungal interference. Always check that the packaging is intact to maintain the sterility of the solution before use. -
Question:
Can I use PBS for washing tissue samples?
Answer: Yes, PBS is commonly used for washing tissue samples before further processing or analysis. Its saline composition helps maintain osmotic balance, preventing tissue distortion during washing. This is particularly important when preparing samples for histological examination or when conducting assays that require intact tissue morphology. Many researchers utilize PBS to wash samples thoroughly, ensuring accurate results in subsequent steps. -
Question:
Where can I buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL?
Answer: You can buy Phosphate Buffered Saline, PBS 1X, Sterile, 500 mL at Ubuy in Samoa. Ubuy offers a wide selection of laboratory supplies, ensuring availability and convenience for researchers looking for high-quality PBS solutions. Shopping through Ubuy gives you access to reliable products tailored for scientific applications.
eConLabSupply Buffers Editorial Review
The phosphate-buffered saline solution is a high-quality product that is sterile and ready to use. It is a balanced salt solution that has a pH of 7.4, isotonicity with body fluids and is free of potential contaminants like endotoxins. The product is ideal for use in a range of laboratory applications that include cell culture, immunology and biochemistry. The PBS 1X solution can be used as a diluent, a buffer or as a washing solution, which makes it extremely versatile. Customer Feedback: Customers have been highly satisfied with this product, citing its convenience and ease of use as major advantages. They have found that the product is reliable and Consistent, which is essential for laboratory experiments. Additionally, the product has met expectations in terms of purity and sterility. Customers have also found that the product is competitively priced for the quality and quantity that it offers.
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Pros
- High-quality, sterile product
- Balanced salt solution with a pH of 7.4 and isotonicity
- Versatile product that can be used in a range of laboratory applications
- Diluent, buffer, or washing solution
- Reliable and Consistent
- Purity and sterility meet expectations
- Competitive pricing
Product Price History
Important information
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WST 141
Order now and get it around Wednesday, July 08
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Features & Benefits
- Contains a balanced, isotonic salt solution
- Used to wash cells before dissociation
- Transporting cells or tissue
- Diluting cells for counting
- Preparing numerous reagents
- Manufactured under certified 14644 clean room conditions in USA lab