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Item #:
75414405
STE Buffer, Sodium Chloride-Tris-EDTA, 10X Solution, pH 8.0, 1L
Item #:
75414405
WST 297
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High-quality STE buffer solution for DNA resuspension and storage
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What Stands Out
Concentration
This 10X concentrated STE buffer simplifies experimentation by providing a highly effective solution for nucleic acid stabilization and purification, ensuring optimal performance in molecular biology applications.
Stability
Formulated with Sodium Chloride, Tris, and EDTA, this buffer maintains pH 8.0 stability, promoting long shelf-life and consistent results for users in research and diagnostic labs.
Versatile Use
Ideal for various molecular biology techniques, this STE buffer supports applications such as DNA extraction, ensuring compatibility with protocols and enhancing procedural efficiency for researchers.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 1.3 Pounds (590 grams) |
| Volume | 1 Liters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.105 |
| Included Components | Bottle |
| Brand | eCon Lab Supply |
| Item Volume | 1 Liters |
| Package Information | Bottle |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biology techniques like DNA isolation and purification, providing an effective buffering environment.
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Research Laboratories
Useful in various laboratory protocols, enhancing reproducibility and consistency in experiments involving nucleic acids.
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Educational Institutions
Suitable for teaching labs, helping students learn techniques involving DNA and RNA manipulation in a controlled environment.
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Clinical Diagnostics
Not suitable for direct use in clinical diagnostics due to specific reagent requirements and regulatory restrictions.
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Non-Biological Research
May not be effective or necessary for research areas unrelated to molecular biology or nucleic acid work.
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Home Use
Not intended for home experiments, where specialized knowledge and safety protocols may not be adequately followed.
Product Description
STE Buffer, Sodium Chloride-Tris-EDTA, 10X Solution, pH 8.0, 1L
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is STE Buffer, Sodium Chloride-Tris-EDTA, 10X Solution used for?
Answer: STE Buffer is primarily used in molecular biology for applications like nucleic acid extraction and purification. Its unique composition helps maintain the integrity of DNA and RNA during isolation processes, preventing degradation and ensuring optimal results. Researchers often use this buffer in techniques such as PCR and gel electrophoresis, where precise pH and ion concentration are crucial for successful outcomes. -
Question:
How should STE Buffer be stored?
Answer: STE Buffer should be stored at room temperature, away from direct sunlight, to maintain its effectiveness. It's essential to keep the container tightly closed to avoid contamination and degradation. In laboratory settings, many prefer to store it in a designated area with other common buffers, ensuring easy access during experiments while keeping it protected from environmental factors that could affect its quality. -
Question:
Can STE Buffer be diluted for different applications?
Answer: Yes, STE Buffer can be diluted based on the requirements of your specific application. Typically, it's used in a 1X concentration for most protocols. However, when working with highly sensitive samples, researchers might opt for lower concentrations to minimize any potential interactions. Always refer to your specific experimental protocol for the recommended buffer concentration to ensure optimal results. -
Question:
Is STE Buffer suitable for use with all types of nucleic acids?
Answer: STE Buffer is suitable for most nucleic acids, including DNA and RNA, due to its balanced pH and ionic strength. However, specific applications may vary based on the requirements of the downstream analysis. For example, while it works well for general purification, certain sensitive RNA applications might require alternative buffers or additional enzymes. Understanding your project’s specific needs is key to achieving the best results. -
Question:
What is the significance of the pH level in STE Buffer?
Answer: The pH 8.0 level in STE Buffer is significant because it creates an optimal environment for nucleic acid stability and solubility. This pH level helps in neutralizing bases and stabilizing the nucleic acids in solution, facilitating their recovery during extraction processes. For instance, if your experiments involve sensitive RNA, maintaining the right pH using STE Buffer can enhance yields and quality, leading to more reliable data. -
Question:
Can I use STE Buffer for cell lysis?
Answer: While STE Buffer is not primarily designed for cell lysis, it can support lysis when used in conjunction with other lysis agents. When extracting nucleic acids from cells, STE Buffer can help maintain stability once the cells are disrupted. Many researchers use it as a stabilizing solution post-lysis to preserve nucleic acid integrity during purification and downstream applications. -
Question:
What are the benefits of using a 10X solution of STE Buffer?
Answer: Using a 10X concentrated solution of STE Buffer provides flexibility for researchers, allowing them to prepare various dilutions according to experimental needs. This concentrated form means you can store a smaller volume, saving space and making it convenient to use across multiple experiments. Moreover, it helps ensure freshness and effectiveness, as you can prepare only the amount needed for your current application. -
Question:
How long can you store a prepared 1X solution of STE Buffer?
Answer: A prepared 1X solution of STE Buffer can typically be stored for several weeks in a sealed container at 4°C. However, it’s recommended to check for signs of contamination or precipitate before use. Maintaining the solution in a clean environment is vital, especially for sensitive assays, ensuring that it remains effective for subsequent applications without risks of degradation. -
Question:
Is STE Buffer compatible with other buffers?
Answer: Yes, STE Buffer can be compatible with other buffers in molecular biology applications as long as the combined ionic strength and pH are compatible with the samples you're working with. Many labs mix STE Buffer with other agents, such as Tris or EDTA, for specific experimental needs, enhancing buffer performance. Always consider the full scope of your protocol to ensure compatibility and effectiveness in your research. -
Question:
Where can I buy STE Buffer, Sodium Chloride-Tris-EDTA, 10X Solution, pH 8.0, 1L?
Answer: You can purchase STE Buffer, Sodium Chloride-Tris-EDTA, 10X Solution, pH 8.0, 1L from Ubuy in Samoa. Ubuy offers a wide selection of laboratory supplies, ensuring that you have reliable access to essential reagents like STE Buffer for your research.
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WST 297
Order now and get it around Saturday, July 11
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Features & Benefits
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use
- Each production lot is manufactured following stringent Good Manufacturing Practices
- Rigorous quality control tests under certified clean room conditions
- Used in plasmid Kits for plasmid purification and DNA extraction
- Physical Form: Liquid, pH 8.0